The Mouse 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit is an indispensable tool for researchers investigating the role of lipid mediators in inflammation, vascular biology, and cancer. This article explores the science, methodology, and applications of this kit, enriched with trusted resources from educational and governmental websites.
12-HETE is a bioactive lipid molecule derived from arachidonic acid through the lipoxygenase pathway. It serves as a signaling molecule involved in inflammatory responses and pathological processes, such as cancer metastasis and atherosclerosis. To learn more about the biochemistry of 12-HETE, visit PubMed or consult the National Library of Medicine.
For detailed insights into eicosanoid pathways, check resources from the National Institutes of Health and the National Center for Biotechnology Information.
Research Areas
The Mouse 12-HETE ELISA Kit is widely used in the following areas:
- Inflammatory Diseases: Understanding diseases like arthritis and inflammatory bowel disease. Explore resources at NIAID.
- Cancer Research: Investigating the role of lipid mediators in tumor progression. Visit the National Cancer Institute.
- Cardiovascular Studies: Exploring the relationship between 12-HETE and vascular health. See research from the National Heart, Lung, and Blood Institute.
Emerging studies also link 12-HETE to neurodegenerative diseases, as detailed by the National Institute on Aging.
Sample Types
This ELISA kit can measure 12-HETE in plasma, serum, urine, and tissue homogenates. For guidelines on sample handling, refer to the Centers for Disease Control and Prevention and the World Health Organization.
Principles of ELISA
The enzyme-linked immunosorbent assay (ELISA) employs antibodies to detect specific molecules in a sample. Tutorials on ELISA techniques can be found at NIAID.
Components of the Kit
- Antibody-coated plates.
- Standards and controls.
- Detection reagents.
- Washing buffers and diluents.
Standard protocols are available through the Food and Drug Administration.
Steps in the Protocol
- Sample Preparation: Follow NIH’s biological sample handling guidelines.
- Incubation: Add samples and standards to the plate. Learn about assay development from NIST.
- Detection: Add enzyme-labeled antibodies for signal generation. See substrate chemistry details at NSF.
- Analysis: Analyze the results using a microplate reader. Explore analytical standards at the Department of Energy.
Troubleshooting tips for ELISA can be found at ABRF.
Advancing Medical Research
The kit helps uncover mechanisms of diseases, contributing to advances in precision medicine. Explore the All of Us Research Program for more on personalized healthcare.
Bridging Lab and Clinic
It enables translational research, making laboratory discoveries clinically relevant. Learn more from NCATS.
Supporting Global Research
The standardized protocols foster international collaboration. Explore global initiatives at USAID.
Environmental Impacts
Understanding lipid mediators like 12-HETE is essential for studying environmental health. Read about environmental biochemistry at the Environmental Protection Agency.
Public Health Applications
Monitoring 12-HETE levels can offer insights into chronic diseases, infections, and population health trends. Resources from the World Health Organization and CDC provide valuable frameworks for integrating biomarker studies into public health initiatives.
The Mouse 12-HETE ELISA Kit is a cornerstone of research in lipidomics, enabling breakthroughs in multiple scientific disciplines. By following established protocols and leveraging resources from trusted institutions, researchers can unlock deeper insights into health and disease.
For further reading, consult Science.gov, OSHA guidelines, and resources from the National Academies.
By supporting discoveries in inflammatory pathways, cancer biology, and environmental health, this kit continues to advance our understanding of critical biological processes and their implications for global health.
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